Dna Barcoding And Its Effects On The Malaysian Market

There is very little customers can do to ensure that they are purchasing the fish they are paying for. Producers and restaurants owners have a tendency to mislabel their products for their own reasons. The main reason is yet to be determined but there are researchers who have made it their goal to resolve this dispute and show customers what they are truly buying. Too Chin Chin et al. attempt to utilize the DNA barcoding in the fish segment, to assess the frequency of fish mislabeling on the Malaysian market (2015). DNA barcoding tries to standardize scientific classification by using short, single DNA sequence, for example, part of the mitochondrial gene COI to distinguish between species (Larson, 2007). The process begins with extracting DNA and using primers to amplify COI regions from the DNA extracts via PCR. The sequences attained are compared to the available sequences in Bold and GenBank databases to find the mislabeled products. Similarly, a character-based key can also be c onstructed to review the reference sequences for variable nucleotides that can serve as diagnostics for the species you wish to study (Loweinstein, 2009). Along with Too Chin Chin et al., there are other researchers who based their study on the same concept to find mislabeled seafood products in certain parts of the world. Dana Miller et al. examined seafood fraud in two types of Whitefish in Europe. Although, the motivation for these works are to find mislabeling in products using the standard